4Q0H
Deinococcus radiodurans BphP PAS-GAF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-30 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.68880 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 89.536, 51.608, 80.633 |
| Unit cell angles | 90.00, 116.32, 90.00 |
Refinement procedure
| Resolution | 36.137 - 1.157 |
| R-factor | 0.1312 |
| Rwork | 0.131 |
| R-free | 0.15710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.328 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.157 |
| Number of reflections | 111918 |
| Completeness [%] | 98.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 296 | 10% PEG 3350, 19% isopropanol, 5% glycerol, 100 mM citric acid(NaOH) ph 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
