4PZI
Zinc finger region of MLL2 in complex with CpG DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.736, 41.005, 55.508 |
| Unit cell angles | 90.00, 99.24, 90.00 |
Refinement procedure
| Resolution | 34.910 - 2.150 |
| R-factor | 0.228 |
| Rwork | 0.226 |
| R-free | 0.27280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | structure of isomorphous crystal was solved by molecular replacement using currently unpublished models of same protein and DNA respectively. |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.434 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.2.14) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.360 | 35.360 | 2.220 |
| High resolution limit [Å] | 2.150 | 8.870 | 2.150 |
| Rmerge | 0.066 | 0.024 | 1.130 |
| Total number of observations | 414 | 2711 | |
| Number of reflections | 8659 | ||
| <I/σ(I)> | 14.3 | 52.6 | 1.4 |
| Completeness [%] | 99.8 | 95 | 100 |
| Redundancy | 3.7 | 3.2 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 291 | 25% PEG-3350, 0.2 M ammonium sulfate, 0.1 M TRIS, pH 8.5, temperature 291K |
