4PY5

Thermovibrio ammonificans RNase H3 in complex with 19-mer RNA/DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-01-31
Detector	PSI PILATUS 6M
Wavelength(s)	0.91841
Spacegroup name	P 21 21 21
Unit cell lengths	58.449, 67.661, 107.514
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.090 - 2.100
R-factor	0.1857
Rwork	0.183
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.074
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (AutoSol)
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.230
High resolution limit [Å]	2.100	2.100
Rmerge	0.069	0.564
Number of reflections	25396
<I/σ(I)>		2.66
Completeness [%]	99.3	95.7
Redundancy		4.27

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	0.2 M AmSO4, 0.1 M BisTris, 25% PEG 3350, 10 mM spermine tetrahydrochloride, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K