4PXJ
Crystallographic structure of the LZII fragment (anti-parallel orientation) from JIP3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-26 |
| Detector | PSI PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 137.826, 63.040, 50.219 |
| Unit cell angles | 90.00, 97.95, 90.00 |
Refinement procedure
| Resolution | 38.670 - 2.060 |
| Rwork | 0.180 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.620 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER (2.5.5) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 43.136 |
| High resolution limit [Å] | 2.060 |
| Number of reflections | 26642 |
| Completeness [%] | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 291 | 1.6 M SAM, 0.1 M citrate pH 5.5, 2 % PEG 400, VAPOR DIFFUSION, temperature 291K |
