4PWK
Crystal structure of V30M mutant human transthyretin complexed with dihydroguaiaretic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-27 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 43.736, 85.217, 64.648 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.340 - 1.590 |
| R-factor | 0.19713 |
| Rwork | 0.195 |
| R-free | 0.23142 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pwe |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.422 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.340 | 1.630 |
| High resolution limit [Å] | 1.590 | 1.600 |
| Rmerge | 0.071 | 0.416 |
| Number of reflections | 29499 | |
| <I/σ(I)> | 19.2 | 2 |
| Completeness [%] | 93.8 | 85.2 |
| Redundancy | 4.8 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 10-30% PEG400, 0.1M HEPES, 0.2M CaCl2, 6mM dihydroguaiaretic acid, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
