4PVZ

Structure of yeast importin a bound to the membrane protein Nuclear Localization Signal sequence of INM protein Heh2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-21
Detector	ADSC QUANTUM 270r
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	49.470, 105.320, 224.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.545 - 2.500
R-factor	0.1925
Rwork	0.191
R-free	0.22630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ee5
RMSD bond length	0.006
RMSD bond angle	0.981
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	40670
<I/σ(I)>	22.5	3.5
Completeness [%]	97.0	97.1
Redundancy	4.4	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	100 mM ammonium acetate, 20% PEG 8000, 100 mM BisTris, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	6	298	100 mM ammonium acetate, 20% PEG 8000, 100 mM BisTris, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K