4PUV

URATE OXIDASE DI-AZIDE complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-01-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.938
Spacegroup name	I 2 2 2
Unit cell lengths	79.180, 94.920, 104.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.300
R-factor	0.1762
Rwork	0.176
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4oqc
RMSD bond length	0.030
RMSD bond angle	0.075
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SHELXDE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.340
High resolution limit [Å]	1.300	1.300
Rmerge	0.072	0.217
Number of reflections	66686
Completeness [%]	98.8	99
Redundancy	4.7	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH METHOD	8.5	291	MIXING TWO SOLUTIONS A & B: SOLUTION A: PROTEIN (20 MG/ML) TRIS ACETATE BUFFER 0.05 M PH=8.5. SOLUTION B: TRIS ACETATE BUFFER 0.05 M + SODIUM AZIDE 0.3M. CRYSTALS HARVESTED 2 MONTHS AFTER CRYSTALLIZATION (AGED CRYSTALS), BATCH METHOD, temperature 291K