4PUH
Oxidized BolA2 from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979733 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.640, 81.419, 33.112 |
| Unit cell angles | 90.00, 108.62, 90.00 |
Refinement procedure
| Resolution | 31.378 - 1.898 |
| R-factor | 0.1788 |
| Rwork | 0.177 |
| R-free | 0.20420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pug |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.041 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.710 | 2.000 |
| High resolution limit [Å] | 1.898 | 1.898 |
| Rmerge | 0.060 | 0.258 |
| Number of reflections | 11470 | |
| <I/σ(I)> | 14.2 | 4 |
| Completeness [%] | 97.1 | 80.8 |
| Redundancy | 3.7 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Oil microbatch method | 8.5 | 278 | 30% PEG1000, pH 8.5, Oil microbatch method, temperature 278K |
