4PUG
BolA1 from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979733 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 30.088, 179.875, 66.818 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.969 - 1.998 |
| R-factor | 0.2007 |
| Rwork | 0.199 |
| R-free | 0.22580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pui |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.849 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.969 | 2.110 |
| High resolution limit [Å] | 1.998 | 2.000 |
| Rmerge | 0.102 | 0.351 |
| Number of reflections | 12798 | |
| <I/σ(I)> | 11.3 | 4.1 |
| Completeness [%] | 99.8 | 98.9 |
| Redundancy | 4.6 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Oil microbatch method | 278 | 18% PEG4000, 6% 2-propanol, 6% 2.5 hexanediol, 4% xylitol and 0.07 M sodium acetate, Oil microbatch method, temperature 278K |
