4PTF
Ternary crystal structure of yeast DNA polymerase epsilon with template G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2009-06-25 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0032 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 147.293, 68.478, 149.079 |
| Unit cell angles | 90.00, 109.60, 90.00 |
Refinement procedure
| Resolution | 46.812 - 2.809 |
| R-factor | 0.1864 |
| Rwork | 0.183 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4m8o |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.049 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.063 | 0.340 |
| Number of reflections | 34036 | |
| <I/σ(I)> | 25.8 | 3.52 |
| Completeness [%] | 98.9 | 83.9 |
| Redundancy | 3.7 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 12% PEG5000 MME, 25 mM magnesium acetate, 1% DMSO, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
