4PSO

Crystal structure of apeThermo-DBP-RP2 bound to ssDNA dT10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-17
Detector	RAYONIX MX-225
Wavelength(s)	0.91841
Spacegroup name	P 21 21 21
Unit cell lengths	120.488, 190.277, 89.786
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.932 - 2.900
R-factor	0.2693
Rwork	0.267
R-free	0.30910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4psn
RMSD bond length	0.004
RMSD bond angle	0.909
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1389))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.000
High resolution limit [Å]	2.900	2.900
Completeness [%]	99.8	99.9
Redundancy	4.5	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	291	200 mM NaOAc, pH 4.3, 1.3 M NaH2PO4 and 0.7 M K2HPO4, VAPOR DIFFUSION, SITTING DROP, temperature 291K