4PSN
Crystal structure of apeThermo-DBP-RP2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-07 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.240, 108.640, 154.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.727 - 2.050 |
| R-factor | 0.1811 |
| Rwork | 0.179 |
| R-free | 0.21590 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.082 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | HKL2Map |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.4 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1 M imidazole, pH 8.0, 0.4 M NaH2PO4, 1.6 M K2HPO4, 0.2 M NaCl and 0.25 M glycine, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
