4PSB

Crystal Structure of Phytohormone Binding Protein from Vigna radiata in complex with gibberellic acid (GA3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-09-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97551
Spacegroup name	C 1 2 1
Unit cell lengths	34.380, 54.290, 71.090
Unit cell angles	90.00, 98.70, 90.00

Refinement procedure

Resolution	35.140 - 1.420
R-factor	0.1543
Rwork	0.152
R-free	0.20390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2flh
RMSD bond length	0.019
RMSD bond angle	1.911
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.140	35.140	1.460
High resolution limit [Å]	1.420	6.350	1.420
Rmerge	0.079	0.043	0.520
Number of reflections	24350
<I/σ(I)>	9.2	20.51	1.98
Completeness [%]	99.5	99.7	99.3
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	292	0.1 M MMT buffer pH 4.0, 25% PEG 1500, VAPOR DIFFUSION, HANGING DROP, temperature 292K