4PQK
C-Terminal domain of DNA binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 64.220, 93.668, 100.806 |
| Unit cell angles | 107.67, 108.30, 84.93 |
Refinement procedure
| Resolution | 46.625 - 3.401 |
| R-factor | 0.2409 |
| Rwork | 0.237 |
| R-free | 0.27540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.215 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.460 |
| High resolution limit [Å] | 3.400 | 9.210 | 3.400 |
| Rmerge | 0.116 | 0.057 | 0.241 |
| Number of reflections | 28157 | ||
| <I/σ(I)> | 10 | ||
| Completeness [%] | 94.9 | 95.3 | 87 |
| Redundancy | 2.4 | 2.8 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 20% PEG 8000, 0.1 M MES pH 6.5 and 0.2 Calcium Acetate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
