4POE

Urate oxidase co-crystallized with uric acid and azide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-01-29
Detector	DECTRIS PILATUS 6M
Spacegroup name	I 2 2 2
Unit cell lengths	79.524, 95.240, 104.577
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.000 - 1.070
R-factor	0.185
Rwork	0.185
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	3l8w
RMSD bond length	0.030
RMSD bond angle	0.076
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.600	1.120
High resolution limit [Å]	1.050	1.050
Rmerge	0.068	0.325
Number of reflections	148909
<I/σ(I)>		2
Completeness [%]	99.3	54.8
Redundancy	4.4	3.15

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5	291	MIXING TWO SOLUTIONS A AND B: SOLUTION A: PROTEIN AT 20MG/ML IN 50MM TRIS-ACETATE BUFFER, + 0.3M SODIUM AZIDE SATURATED BY URIC ACID (IN PRESENCE OF THE SOLID), PH 8.5 SOLUTION B: PEG-8000 18% IN TRIS BUFFER, BATCH METHOD, temperature 291K