4PO1
Mycobacterium tuberculosis RecA glycerol bound room temperature structure IIC-RT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-05-15 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 61 |
| Unit cell lengths | 108.340, 108.340, 72.540 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 21.150 - 3.400 |
| R-factor | 0.17113 |
| Rwork | 0.165 |
| R-free | 0.22868 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g19 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.076 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.390 | 3.580 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.337 | 0.872 |
| Number of reflections | 6799 | |
| <I/σ(I)> | 7.6 | 2.6 |
| Redundancy | 6 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 298 | 15% PEG3350, 10% PEG5000 MME, 0.2M AMMONIUM ACETATE, 0.1M SODIUM CITRATE , pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
