4PNS

Crystal Structure of human Tankyrase 2 in complex with INH2BP.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2009-08-12
Detector	ADSC QUANTUM 210
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	73.920, 79.500, 153.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.250 - 1.650
R-factor	0.1885
Rwork	0.188
R-free	0.20920
RMSD bond length	0.010
RMSD bond angle	1.010
Data reduction software	XDS (2005/3)
Refinement software	BUSTER-TNT (BUSTER 2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.250	44.250	1.680
High resolution limit [Å]	1.650	4.700	1.650
Rmerge	0.063	0.034	0.563
Total number of observations	737631
Number of reflections	101225	5090	3608
<I/σ(I)>	17.17	46.91	1.86
Completeness [%]	92.4	99.4	94.4
Redundancy	6.41	6.67	4.08

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.2M NaCl, 0.1M Sodium, Hepes buffer at pH7.5, 12-15% iso-propanol