4PNO
Escherichia coli Hfq-RNA complex at 0.97 A Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-12-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.885611 |
Spacegroup name | P 6 |
Unit cell lengths | 61.110, 61.110, 27.860 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.555 - 0.970 |
R-factor | 0.1437 |
Rwork | 0.142 |
R-free | 0.16390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hk9 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.866 |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.920 | 1.005 |
High resolution limit [Å] | 0.970 | 0.970 |
Rmerge | 0.033 | |
Number of reflections | 68880 | |
<I/σ(I)> | 26 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.2 | 8.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 12% (w/v) PEG 3350; 0.1M Hepes/NaOH, pH 8.0; 0.25M KSCN |