4PN9
A de novo designed hexameric coiled coil CC-Hex2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2013-07-09 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 59.320, 125.530, 58.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.657 - 2.200 |
| R-factor | 0.2088 |
| Rwork | 0.206 |
| R-free | 0.25780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | search model was in silico |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.098 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.660 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.082 | |
| Number of reflections | 11120 | |
| <I/σ(I)> | 10.7 | |
| Completeness [%] | 98.2 | 98.7 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292.15 | 0.1 M Na HEPES, 4.3 M Sodium Chloride |
