4PN1
Structure of S. pombe Pct1 RNA triphosphatase in complex with the Spt5 CTD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 95.603, 251.542, 75.397 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.362 - 2.803 |
| R-factor | 0.1942 |
| Rwork | 0.191 |
| R-free | 0.24770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pn0 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.681 |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.113 | 0.430 |
| Number of reflections | 49568 | |
| <I/σ(I)> | 13.8 | 2.6 |
| Completeness [%] | 99.1 | 97.7 |
| Redundancy | 4.9 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 3.0 M sodium formate cryo-protected in 3.2 M sodium formate and 20% (v/v) ethylene glycol |
