4PMC
Crystal structure of Staphylcoccal nuclease variant Delta+PHS I72K V74K at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2014-04-28 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.880, 59.889, 73.765 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.880 - 1.650 |
| R-factor | 0.183 |
| Rwork | 0.182 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.780 |
| Data reduction software | SAINT |
| Data scaling software | APEX |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.670 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.018 | 0.292 |
| Number of reflections | 17697 | |
| <I/σ(I)> | 47.22 | 3.3 |
| Completeness [%] | 99.9 | 98.6 |
| Redundancy | 35.43 | 4.97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 25% MPD, 25mM potassium phosphate, calcium chloride, pdTp |
