4PLL

Structure of the chromodaomain of MRG2 in complex with H3K36me3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791
Spacegroup name	P 61
Unit cell lengths	109.157, 109.157, 29.977
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.575 - 2.600
R-factor	0.2437
Rwork	0.240
R-free	0.27930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.330
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.094	0.480
Number of reflections	6489
<I/σ(I)>	31	2.4
Completeness [%]	99.1	97.4
Redundancy	6.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	290	0.1M MES pH 6.5, 30% polyethylene glycol monomethyl ether 5000, 0.2M Ammonium sulfate