4PLB

Crystal Structure of S.A. gyrase-AM8191 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-07-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 61
Unit cell lengths	93.310, 93.310, 410.197
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.120 - 2.690
R-factor	0.1788
Rwork	0.176
R-free	0.22000
RMSD bond length	0.010
RMSD bond angle	1.210
Refinement software	BUSTER (2.9.7)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	410.200	2.840
High resolution limit [Å]	2.690	2.690
Rmerge	0.083	0.542
Number of reflections	55628
<I/σ(I)>	23.3	4.2
Completeness [%]	100.0	100
Redundancy	10.4	10.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	15.33 % PEG MME 5K, 50 mM Bis-Tris pH 6.0