4PLA

Crystal structure of phosphatidyl inositol 4-kinase II alpha in complex with ATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-25
Detector	RAYONIX MX-225
Wavelength(s)	0.91841
Spacegroup name	P 21 21 2
Unit cell lengths	104.744, 79.539, 78.704
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.838 - 2.771
R-factor	0.2135
Rwork	0.211
R-free	0.25110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.421
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	36.840
High resolution limit [Å]	2.770
Rmerge	0.159
Number of reflections	16256
<I/σ(I)>	23.81
Completeness [%]	93.9
Redundancy	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	8% PEG 4000, 150 mM (NH4)2SO4, 100 mM MES, pH = 6.5, 10 mM betaine