4PL7
Structure of Komagataella pastoris actin-thymosin beta4 hybrid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.508, 139.495, 56.485 |
| Unit cell angles | 90.00, 116.21, 90.00 |
Refinement procedure
| Resolution | 19.831 - 2.300 |
| R-factor | 0.1982 |
| Rwork | 0.195 |
| R-free | 0.25790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yag chain A |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.322 |
| Data reduction software | SCALEPACK |
| Phasing software | PHASER (2.5.5) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 4.720 | 2.200 |
| Rmerge | 0.109 | 0.080 | 0.373 |
| Total number of observations | 132268 | ||
| Number of reflections | 37614 | ||
| <I/σ(I)> | 10.6 | ||
| Completeness [%] | 94.5 | 99.1 | 71.8 |
| Redundancy | 3.5 | 3.7 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | 0.1 M sodium acetate, 15% (w/v) PEG 4000 |
