4PKI

Complex of ATP-actin With the C-terminal Actin-Binding Domain of Tropomodulin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-22
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0781
Spacegroup name	P 21 21 21
Unit cell lengths	69.393, 81.211, 170.708
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.000 - 2.300
R-factor	0.1697
Rwork	0.168
R-free	0.20050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1io0
RMSD bond length	0.014
RMSD bond angle	1.431
Data scaling software	HKL-2000
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.440
High resolution limit [Å]	2.300	2.300
Rmerge	0.063	0.570
Number of reflections	43679
<I/σ(I)>	32.8	3.1
Completeness [%]	99.9	100
Redundancy	12.8	12.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	0.25 M sodium chloride, 12% w/v PEG3350