4PK2

tubulin acetyltransferase complex with bisubstrate analog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	193
Detector technology	CCD
Collection date	2013-01-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	37.124, 52.483, 50.285
Unit cell angles	90.00, 106.83, 90.00

Refinement procedure

Resolution	35.535 - 1.350
R-factor	0.1329
Rwork	0.131
R-free	0.16820
RMSD bond length	0.009
RMSD bond angle	1.664
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall
Low resolution limit [Å]	35.535
High resolution limit [Å]	1.347
Number of reflections	40567
<I/σ(I)>	21.8
Completeness [%]	99.8
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	0.1M Tris 8.5, 0.2M Ammonium Acetate, 25% Peg3350