4PJQ
Crystal structure of designed cPPR-polyG protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | F 2 3 |
| Unit cell lengths | 207.543, 207.543, 207.543 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.614 - 3.353 |
| R-factor | 0.1956 |
| Rwork | 0.190 |
| R-free | 0.24750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pjs |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.685 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.614 | 3.620 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Rmerge | 0.057 | 1.011 |
| Number of reflections | 20728 | |
| <I/σ(I)> | 17.8 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.9 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 291.15 | 0.1M BisTris pH 6.7, 22% PEG3350 |
