4PJN

Myosin VI motor domain in the Pi release state, space group P212121 - shortly soaked with PO4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9801
Spacegroup name	P 21 21 21
Unit cell lengths	91.320, 95.750, 103.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.280 - 2.000
R-factor	0.1722
Rwork	0.170
R-free	0.20720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.020
Data reduction software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.125	0.880
Number of reflections	61572
<I/σ(I)>	13.64	1.94
Completeness [%]	99.4	96.8
Redundancy	8.2	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	7% PEG8K, 50mM Tris pH8,5, 1mM TCEP, 3% Glyc?rol, then soaked with 30% Glycerol and finaly soaked few secondes with 100mM NaH2PO4 pH 7.5