4PJ0

Structure of T.elongatus Photosystem II, rows of dimers crystal packing

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	OTHER
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-03-22
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.196
Spacegroup name	P 21 21 21
Unit cell lengths	116.447, 218.889, 302.202
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.123 - 2.437
R-factor	0.2179
Rwork	0.216
R-free	0.25560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ARC
RMSD bond length	0.007
RMSD bond angle	0.965
Refinement software	PHENIX ((phenix.refine: 1.9_1690))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.120	2.524
High resolution limit [Å]	2.440	2.440
Rmerge	0.094	0.698
Number of reflections	282213
<I/σ(I)>	14.7	1.93
Completeness [%]	98.4	89.5
Redundancy	6	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	293	Batch crystallisation: 2 mM Chl a equivalent of PSIIcc mixed 1:1 with: 0.1 M TRIS (pH 7.5), 0.1 M (NH4)2SO4, 15 - 18 % PEG 5000 MME, post-crystallisation dehydration