4PIR

X-ray structure of the mouse serotonin 5-HT3 receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-12-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	121.542, 187.087, 240.554
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.999 - 3.500
R-factor	0.2194
Rwork	0.217
R-free	0.25740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.641
Refinement software	PHENIX ((phenix.refine: 1.9_1685))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.690
High resolution limit [Å]	3.500	3.500
Rmerge		1.329
Number of reflections	69760
<I/σ(I)>	10.1
Completeness [%]	99.8	99.9
Redundancy	5.6	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		285	20-25% PEG 10000 0.1 M Na2SO4 0.1 M Tris pH 8.5