4PHY

Functional conservation despite structural divergence in ligand-responsive RNA switches

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	173
Detector technology	IMAGE PLATE
Collection date	2013-09-06
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54
Spacegroup name	P 63 2 2
Unit cell lengths	79.611, 79.611, 100.940
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.375 - 3.100
R-factor	0.2173
Rwork	0.215
R-free	0.26790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.070
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.900	3.210
High resolution limit [Å]	3.100	3.100
Number of reflections	3167
<I/σ(I)>	22.28	2.5
Completeness [%]	88.2	56.4
Redundancy	28	15.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	KCl, Magnesium Acetate, PEG 8K