4PHP
Structure of human DNA polymerase beta complexed with T in the template base paired with incoming non-hydrolyzable GTP and MANGANESE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-05-01 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.701, 79.327, 55.474 |
Unit cell angles | 90.00, 107.58, 90.00 |
Refinement procedure
Resolution | 19.833 - 2.600 |
R-factor | 0.185 |
Rwork | 0.181 |
R-free | 0.26160 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.997 |
Data reduction software | DENZO |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.600 |
Number of reflections | 12915 |
<I/σ(I)> | 9.3 |
Completeness [%] | 99.9 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 14%?23% PEG3400, and 350 mM sodium acetate in 50 mM imidazole (pH 7.5) |