4PHG

Crystal structure of Ypt7 covalently modified with GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-09-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	32.970, 53.160, 46.910
Unit cell angles	90.00, 106.35, 90.00

Refinement procedure

Resolution	45.010 - 1.900
R-factor	0.17758
Rwork	0.175
R-free	0.23398
RMSD bond length	0.019
RMSD bond angle	1.910
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.010	2.000
High resolution limit [Å]	1.900	1.900
Number of reflections	12310
<I/σ(I)>	11.15
Completeness [%]	99.5
Redundancy	3.33

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	20% (w/v) PEG3350, 0.2 M LiCl, 10mM Yttrium(III)-chlorid