4PH5

Structure of human DNA polymerase beta complexed with a nicked DNA containing a AC at N-1 position and GC at N position

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-10-29
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	50.613, 80.377, 55.294
Unit cell angles	90.00, 107.61, 90.00

Refinement procedure

Resolution	20.000 - 2.550
R-factor	0.1945
Rwork	0.191
R-free	0.26120
RMSD bond length	0.003
RMSD bond angle	0.629
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.550
Number of reflections	13392
<I/σ(I)>	3.8
Completeness [%]	98.2
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	14%?23% PEG3400, and 350 mM sodium acetate in 50 mM imidazole (pH 7.5)