4PGX

Structure of human DNA polymerase beta complexed with G in the template base paired with incoming non-hydrolyzable TTP and MANGANESE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97648
Spacegroup name	P 1 21 1
Unit cell lengths	50.969, 79.796, 55.635
Unit cell angles	90.00, 107.37, 90.00

Refinement procedure

Resolution	19.970 - 2.077
R-factor	0.1854
Rwork	0.183
R-free	0.22650
RMSD bond length	0.004
RMSD bond angle	1.078
Data reduction software	DENZO
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.077
Number of reflections	25542
<I/σ(I)>	21
Completeness [%]	100.0
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	14%?23% PEG3400, and 350 mM sodium acetate in 50 mM imidazole (pH 7.5)