4PG2
The crystal structure of H-2Db with a S-glutathionylated peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-05-28 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 2 2 21 |
Unit cell lengths | 75.699, 79.099, 85.933 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.860 - 2.800 |
R-factor | 0.21399 |
Rwork | 0.208 |
R-free | 0.26668 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.127 |
Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 16.000 |
High resolution limit [Å] | 2.800 |
Number of reflections | 11701 |
<I/σ(I)> | 10.5 |
Completeness [%] | 87.9 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 0.1M citrate pH7.5, 28% PEG 3350, 0.2M LiSO4 |