4PFW

Crystal structure of mannohexaose bound oligopeptide ABC transporter, periplasmic oligopeptide-binding protein (TM1226) from THERMOTOGA MARITIMA at 2.2 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-05-07
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	99.548, 194.227, 159.425
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.862 - 2.198
R-factor	0.167
Rwork	0.164
R-free	0.21770
RMSD bond length	0.008
RMSD bond angle	1.074
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.198	2.198
Rmerge		0.568
Number of reflections	76389
<I/σ(I)>	15.7	2.4
Completeness [%]	97.2	97.2
Redundancy	3.9	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	2.2 M AmSO4, 0.2 M tri-Li Citrate