4PFU
Crystal structure of mannobiose bound oligopeptide ABC transporter, periplasmic oligopeptide-binding protein (TM1226) from THERMOTOGA MARITIMA at 2.05 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-03-12 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 100.911, 193.867, 159.513 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.467 - 2.049 |
| R-factor | 0.1752 |
| Rwork | 0.173 |
| R-free | 0.20810 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.073 |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.073 |
| High resolution limit [Å] | 2.049 | 2.049 |
| Rmerge | 0.584 | |
| Number of reflections | 96558 | |
| <I/σ(I)> | 18.2 | 3.5 |
| Completeness [%] | 98.5 | 96.1 |
| Redundancy | 5.7 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | 2.2 M AmSO4, 0.2 M tri-Li Citrate |
