4PFQ
Crystal structure of hypoxanthine phosphoribosyltransferase from Brachybacterium faecium DSM 4810, NYSGRC Target 029763.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-09 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.210, 123.587, 123.285 |
| Unit cell angles | 90.00, 90.45, 90.00 |
Refinement procedure
| Resolution | 33.310 - 2.100 |
| R-factor | 0.176 |
| Rwork | 0.173 |
| R-free | 0.21300 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.887 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 5.700 | 2.100 |
| Rmerge | 0.117 | 0.086 | 0.644 |
| Total number of observations | 408245 | ||
| Number of reflections | 86164 | ||
| <I/σ(I)> | 12.6 | ||
| Completeness [%] | 95.9 | 98.9 | 92.1 |
| Redundancy | 4.7 | 5.3 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.2 M Mg-chloride, 20% PEG 3350 |
