4PFK

PHOSPHOFRUCTOKINASE. STRUCTURE AND CONTROL

Experimental procedure

Spacegroup name	I 2 2 2
Unit cell lengths	122.500, 84.100, 61.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.400
R-factor	0.169
RMSD bond length	0.011
RMSD bond angle	0.041
Refinement software	PROLSQ

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.3 *		THE CRYSTALS USED IN THIS STUDY WERE GROWN FROM POTASSIUM PHOSPHATE WITH 2 MILLIMOLAR FRUCTOSE-6-PHOSPHATE. FOR THIS LIGANDED STRUCTURE, THE CRYSTALS WERE SOAKED IN FRUCTOSE-6-PHOSPHATE (F6P) AND ADP/MG IN POTASSIUM TARTRATE. F6P 323 AND THE EFFECTOR SITE ADP 326 ARE TREATED AS FULLY OCCUPIED WHILE ADP 324 IS TREATED AS HALF OCCUPIED.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	potassium phosphate	2 (M)
2	1	1	F6P	2 (mM)