4PFE

Crystal structure of vsfGFP-0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-15
Detector	ADSC QUANTUM 315
Wavelength(s)	1.075
Spacegroup name	P 41 21 2
Unit cell lengths	83.426, 83.426, 228.691
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.160 - 2.603
R-factor	0.2013
Rwork	0.199
R-free	0.25530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3k1k
RMSD bond length	0.004
RMSD bond angle	1.045
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.160	2.696
High resolution limit [Å]	2.603	2.603
Rmerge	0.112	0.603
Number of reflections	25661
<I/σ(I)>	20.97	3.9
Completeness [%]	100.0	99.48
Redundancy	32	28.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.1M Sodium acetate pH 4.6 30 %(v/v) PEG 400, 20mM Hepes pH 7.5, 150mM NaCl