4PEG

Dbr1 in complex with guanosine-5'-monophosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-04
Detector	ADSC QUANTUM 315
Wavelength(s)	1.2823
Spacegroup name	P 21 21 21
Unit cell lengths	72.831, 141.499, 212.942
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.538 - 2.000
R-factor	0.1989
Rwork	0.197
R-free	0.22910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.902
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.840	2.110
High resolution limit [Å]	2.000	2.000
Rmerge		0.499
Number of reflections	143800
<I/σ(I)>	13	3.1
Completeness [%]	96.7	96.4
Redundancy	4.9	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	295	0.2 M lithium sulfate, 0.1 M bis-tris, 25% PEG 3350, 8.5 % glycerol, 1.0 mM guanosine-5'-monophosphate, 2.0 mM manganese sulfate