4PDO
Structure of Ephrin type-A receptor 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 32 |
| Unit cell lengths | 92.461, 92.461, 75.836 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.902 - 2.104 |
| R-factor | 0.1615 |
| Rwork | 0.160 |
| R-free | 0.18380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mqb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.016 |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 15.000 | 15.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 5.600 | 2.100 |
| Rmerge | 0.086 | 0.051 | 0.444 |
| Total number of observations | 197356 | ||
| Number of reflections | 41909 | ||
| <I/σ(I)> | 9.3 | ||
| Completeness [%] | 99.8 | 97.6 | 100 |
| Redundancy | 4.7 | 4 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.8 M Na/K L(+)-tartrate, 0.1 M Tris-Cl pH 8.5, 1% (w/v) PEGMME 5000 |
