4PD1

Structure of gephyrin E domain with Glycine-beta receptor peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-29
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9184
Spacegroup name	I 2 2 2
Unit cell lengths	85.660, 100.060, 117.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.472 - 1.975
R-factor	0.1605
Rwork	0.159
R-free	0.19550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fu3
RMSD bond length	0.011
RMSD bond angle	1.297
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	36.472
High resolution limit [Å]	1.975
Rmerge	0.111
Number of reflections	35783
<I/σ(I)>	17.7
Completeness [%]	100.0
Redundancy	14.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	0.1 M sodium citrate, pH 4.5, 28-34 % 2-methyl-2-4-pentanediol