4PCZ

Crystal structure of a complex between R247G LlFPG mutant and a THF containing DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-09-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98011
Spacegroup name	P 41 21 2
Unit cell lengths	91.723, 91.723, 142.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.940 - 1.700
R-factor	0.158
Rwork	0.157
R-free	0.18150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pm5
RMSD bond length	0.019
RMSD bond angle	1.825
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.940	1.790
High resolution limit [Å]	1.700	1.700
Rmerge	0.095	0.411
Number of reflections	65739
<I/σ(I)>	8.4	2.6
Completeness [%]	97.4	88.2
Redundancy	3.6	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	HEPES, SODIUM CITRATE