4PCJ
Modifications to toxic CUG RNAs induce structural stability and rescue mis-splicing in Myotonic Dystrophy
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 85 |
| Detector technology | CCD |
| Collection date | 2012-11-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | H 3 |
| Unit cell lengths | 69.705, 69.705, 67.797 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.600 - 1.900 |
| R-factor | 0.19963 |
| Rwork | 0.196 |
| R-free | 0.26770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fnj |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.926 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.600 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.051 | 0.440 |
| Number of reflections | 9697 | |
| <I/σ(I)> | 22.7 | 2.4 |
| Completeness [%] | 96.9 | 89.6 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | MgSO4, Tris (pH 8.5), 1,6-hexanediol |
