4PCG
Structure of Human Polyomavirus 6 (HPyV6) VP1 pentamer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 183.900, 89.440, 125.270 |
| Unit cell angles | 90.00, 131.26, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.14926 |
| Rwork | 0.148 |
| R-free | 0.17438 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Human Polyomavirus 7 (HPyV7) VP1 pentamer |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.381 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 140450 | |
| <I/σ(I)> | 14.8 | 2.8 |
| Completeness [%] | 99.2 | 96.4 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 100 mM Tris pH 8.5, 200 mM NaSCN, 13.3% (w/v) PEG 3350 |
