4PC1

Elongation Factor Tu:Ts complex with a bound phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.5
Synchrotron site	SRS
Beamline	PX9.5
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1995-08-10
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.9215
Spacegroup name	P 21 21 21
Unit cell lengths	73.510, 109.770, 195.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	68.786 - 1.950
R-factor	0.1757
Rwork	0.174
R-free	0.20770
Starting model (for MR)	1tui
RMSD bond length	0.013
RMSD bond angle	1.350
Refinement software	PHENIX ((phenix.refine: dev_1593))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.800	2.000
High resolution limit [Å]	1.950	1.950
Rmerge		0.658
Number of reflections	118029
<I/σ(I)>	11.6	1.8
Completeness [%]	96.8	97.2
Redundancy	2.7	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	292	100 mM Hepes,22% PEG 6000, 150mM NaCl, 10% glycerol