4PC0
Structure of the human RbAp48-MTA1(670-711) complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-11-14 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.250, 123.230, 87.340 |
| Unit cell angles | 90.00, 103.39, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.2065 |
| Rwork | 0.204 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xu7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.041 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.610 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.357 | |
| Number of reflections | 33957 | |
| <I/σ(I)> | 11.9 | 3.8 |
| Completeness [%] | 91.1 | 100 |
| Redundancy | 6.9 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M calcium acetate and 20% w/v PEG 3350 |
